NO! is a trinket added in the The Binding of Isaac: Afterbirth expansion.
- Prevents activated collectibles from being generated, if possible, from all item pools while this trinket is being held.
- Activated items will start appearing again once the item pool's non-activated items are exhausted or getting very low.
- When the trinket is dropped, the item generation mechanics go back to normal, and active items will be generated normally again.
- Does not affect Libraries until all the books have been collected, as the library pool consists only of activated items.
- Fixed boss drops and machines are not affected by NO!:
- In runs where many items have been collected/rerolled, the trinket can function as a sort of warning to the player that an item pool will soon be exhausted if activated items begin to show up.
Yum Heart from Super Lust
Mr. Boom from Wrath
Anarchist Cookbook from Pride
Shoop Da Whoop! from Envy
Bob's Rotten Head from Sloth
A Pony and 
White Pony from Horsemen
Head of Krampus from Krampus
IV Bag from blood donation machine
Crystal Ball from fortune teller
NO!'s original appearance before Booster Pack #5.
The Binding of Isaac: Rebirth 
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