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Added in Afterbirth
Challenge number
Isaac App.png
HUD Red Heart.pngHUD Red Heart.pngHUD Red Heart.png
Starting Items
3 Dollar Bill 3 Dollar Bill
Rainbow Baby Rainbow Baby
Added in Afterbirth Rainbow Worm Rainbow Worm
Available rooms
Item Pool Treasure Room.png: no          Item Pool Shop.png: yes
Boss Mom's Heart.png / Boss It Lives.png
D8 Added in Afterbirth D8
Unlocked by
PRIDE DAY! Added in Afterbirth Defeat Mom for the 1st time

PRIDE DAY! is challenge #28. You start off with Rainbow Worm, the 3 Dollar Bill, and Rainbow Baby. Your goal is to defeat Mom's Heart or It Lives. The Rainbow Worm cannot be dropped in this challenge.


This is a rather casual challenge run. Many of the potential effects of the Rainbow Tears end up being beneficial overall. However, certain tears could temporarily be detrimental, such as Fire Mind tears causing self-damage or Proptosis greatly decreasing your range. You will also need to adapt to whatever tear trajectory the Rainbow Worm will give you as well.



  • The name of the challenge "Pride Day" and the rainbow-themed items of the challenge is a reference to the Gay Pride or LGBT Pride community and their signature rainbow flag.

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