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Spirit of the Night is a passive item.
- Grants spectral tears and flight.
- This item belongs to the Leviathan set. Collecting three items from this set will transform Isaac into Leviathan.
- It is the Devil Room equivalent of the Dead Dove.
- The changed texture of the player's body is the same as The Lost's, but recolored.
- Lord of the Pit costs the same amount of Red Hearts as Spirit of the Night, while only granting flight and increased speed. It´s strongly recommended to take Spirit of the Night if the player has the choice.
- The name and Isaac's appearance are a reference to the Magic: the Gathering card.
- The item's icon is the Wiccan symbol for blessing.