Trinkets are a kind of collectible with their designated slot that provide passive bonuses. Only one trinket can be carried at a time and must be dropped if you wish to carry another. Some of these limitations can be bypassed with certain items; 
Mom's Purse and 
Belly Button allow two trinkets to be held at once, and trinkets can be absorbed by 
Smelter, 
Marbles, or Gulp!. Absorbed trinkets do not take up a trinket slot and act as passive effects.
The original Binding of Isaac DLC 'The Wrath of the Lamb' introduced Trinkets as a mechanic and had 33 trinkets. Rebirth has a total of 60 trinkets. Afterbirth added 29 new trinkets, for a total of 89 trinkets. Afterbirth † added 38 new trinkets, for a total of 128 trinkets.
Notes
- It is possible to drop a trinket by holding down the key or button bound to the "Drop" function for three seconds. (Doing this is not possible with the
Tick). - While held, Mom's Box doubles the effect of most trinkets.
- Trinkets appear in a set order based on the run's seed, and each trinket can usually only be encountered once.
- Some trinkets are set drops, such as Mysterious Paper from Divorce Papers and The Left Hand from Ultra Pride, that will appear even if encountered previously.
- Other set drops, such as Swallowed Penny from a urn or Umbilical Cord from a polyp will not appear if already seen.
- If the player spawns enough trinkets, e.g. through Mom's Box, the cycle loops and begins spawning trinkets that were already encountered.
- If the player manages to get two of the same trinket then they can have both by using gulp! on one and then picking up the other.
- Having two of the same trinket doesn't increase the effect.
- Some trinkets are set drops, such as
All Trinkets
Trivia
- The Polaroid was originally a trinket in the original Binding of Isaac, but it was changed into an item.
- Wiggle Worm was an item in the original Binding of Isaac; however, in Rebirth, it was changed into a trinket. Interestingly, these changes keep the count of items and trinkets from the original game the same.
- Afterbirth+'s original trinket count was 29. The Booster Packs added 9 trinkets.
- The Game Squid is the only item that was in the original Binding of Isaac that wasn't implemented into Rebirth, let alone any of its DLCs. The reason is most likely that it was an item that was added as a result of the Eternal Edition update for the original Binding of Isaac, which was implemented a while after the release of the Binding of Isaac: Rebirth.
 The Binding of Isaac: Rebirth
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